Recombinant Human Phenylethanolamine N-methyltransferase
Cat.No. : | PNMT-481H |
Product Overview : | Recombinant human PNMT protein was expressed inE.coliand purified by using conventional chromatography techniques. MW = 30.8 Da (282aa). |
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Species : | Human |
Source : | E.coli |
Tag : | Non |
Description : | PNMT, also known as phenylethanolamine N-methyltransferase, is an enzyme found in the adrenal medulla that catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. |
Amino Acid Sequence : | MSGADRSPNA GAAPDSAPGQ AAVASAYQRF EPRAYLRNNY APPRGDLCNP NGVGPWKLRC LAQTFATGEV SGRTLIDIGS GPTVYQLLSA CSHFEDITMT DFLEVNRQEL GRWLQEEPGA FNWSMYSQHA CLIEGKGECW QDKERQLRAR VKRVLPIDVH QPQPLGAGSP APLPADALVS AFCLEAVSPD LASFQRALDH ITTLLRPGGH LLLIGALEES WYLAGEARLT VVPVSEEEVR EALVRSGYKV RDLRTYIMPA HLQTGVDDVK GVFFAWAQKV GL |
Form : | Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol. |
Purity : | > 95% by SDS – PAGE. |
Concentration : | 1.0 mg/ml (determined by Bradford assay). |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Publications : |
Noncompetitive Inhibition of Indolethylamine N-Methyltransferase by N, N Dimethyltryptamine (DMT) and N, N-Dimethylaminopropyltryptamine (2014)
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Gene Name | PNMT phenylethanolamine N-methyltransferase [ Homo sapiens ] |
Synonyms | PNMT; phenylethanolamine N-methyltransferase; PENT; PNMTase; MGC34570; phenylethanolamine N-methyltransferase; phenylethanolamine N-methylase; noradrenaline N-methyltransferase; EC 2.1.1.28; OTTHUMP00000164271 |
Gene ID | 5409 |
mRNA Refseq | NM_002686 |
Protein Refseq | NP_002677 |
MIM | 171190 |
UniProt ID | P11086 |
Chromosome Location | 17q21-q22 |
Pathway | Metabolic pathways; Tyrosine metabolism |
Function | methyltransferase activity; phenylethanolamine N-methyltransferase activity; transferase activity |
◆ Recombinant Proteins | ||
PNMT-197H | Active Recombinant Human PNMT protein, GST-tagged | +Inquiry |
PNMT-30072TH | Recombinant Human PNMT | +Inquiry |
PNMT-4549R | Recombinant Rat PNMT Protein | +Inquiry |
PNMT-1440HFL | Recombinant Full Length Human PNMT Protein, C-Flag-tagged | +Inquiry |
PNMT-328H | Recombinant Human PNMT | +Inquiry |
◆ Cell & Tissue Lysates | ||
PNMT-3075HCL | Recombinant Human PNMT 293 Cell Lysate | +Inquiry |
Noncompetitive Inhibition of Indolethylamine- N -methyltransferase by N , N -Dimethyltryptamine and N , N -Dimethylaminopropyltryptamine
Journal: Biochemistry PubMed ID: 24730580 Data: 2015/4/14
Authors: Uyen B. Chu, Sevahn K. Vorperian, Arnold E. Ruoho
Article Snippet:Histidine-tagged human indolethylamine- N -methyltransferase was obtained from the Structural Genomics Consortium (University of Toronto, Toronto, ON).Histidine-tagged human indolethylamine- N -methyltransferase was obtained from the Structural Genomics Consortium (University of Toronto, Toronto, ON).. Recombinant human phenylethanolamine- N -methyltransferase (hPNMT) and nicotinamide- N -methyltransferase (hNNMT) were purchased from Creative BioMart (Shirley, NY).. All reagents for the hPNMT assay were prepared in 50 mM Tris-HCl buffer (pH 8.5) unless stated otherwise, excluding PDAT, which was dissolved in dimethyl sulfoxide (DMSO).All reagents for the hPNMT assay were prepared in 50 mM Tris-HCl buffer (pH 8.5) unless stated otherwise, excluding PDAT, which was dissolved in dimethyl sulfoxide (DMSO).
Not For Human Consumption!
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