Recombinant Human Phenylethanolamine N-methyltransferase

• Cat.No.: PNMT-481H
• Product Overview: Recombinant human PNMT protein was expressed inE.coliand purified by using conventional chromatography techniques. MW = 30.8 Da (282aa).

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: PNMT, also known as phenylethanolamine N-methyltransferase, is an enzyme found in the adrenal medulla that catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production.
• Amino Acid Sequence: MSGADRSPNA GAAPDSAPGQ AAVASAYQRF EPRAYLRNNY APPRGDLCNP NGVGPWKLRC LAQTFATGEV SGRTLIDIGS GPTVYQLLSA CSHFEDITMT DFLEVNRQEL GRWLQEEPGA FNWSMYSQHA CLIEGKGECW QDKERQLRAR VKRVLPIDVH QPQPLGAGSP APLPADALVS AFCLEAVSPD LASFQRALDH ITTLLRPGGH LLLIGALEES WYLAGEARLT VVPVSEEEVR EALVRSGYKV RDLRTYIMPA HLQTGVDDVK GVFFAWAQKV GL
• Form: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol.
• Purity: > 95% by SDS – PAGE.
• Concentration: 1.0 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Publications:
  Noncompetitive Inhibition of Indolethylamine N-Methyltransferase by N, N Dimethyltryptamine (DMT) and N, N-Dimethylaminopropyltryptamine (2014)

Gene Information

• Gene Name: PNMT phenylethanolamine N-methyltransferase [ Homo sapiens ]
• Synonyms: PNMT; phenylethanolamine N-methyltransferase; PENT; PNMTase; MGC34570; phenylethanolamine N-methyltransferase; phenylethanolamine N-methylase; noradrenaline N-methyltransferase; EC 2.1.1.28; OTTHUMP00000164271
• Gene ID: 5409
• mRNA Refseq: NM_002686
• Protein Refseq: NP_002677
• MIM: 171190
• UniProt ID: P11086
• Chromosome Location: 17q21-q22
• Pathway: Metabolic pathways; Tyrosine metabolism
• Function: methyltransferase activity; phenylethanolamine N-methyltransferase activity; transferase activity

Citation

Noncompetitive Inhibition of Indolethylamine- N -methyltransferase by N , N -Dimethyltryptamine and N , N -Dimethylaminopropyltryptamine

Journal: Biochemistry    PubMed ID: 24730580    Data: 2015/4/14

Authors: Uyen B. Chu, Sevahn K. Vorperian, Arnold E. Ruoho

Article Snippet:Histidine-tagged human indolethylamine- N -methyltransferase was obtained from the Structural Genomics Consortium (University of Toronto, Toronto, ON).Histidine-tagged human indolethylamine- N -methyltransferase was obtained from the Structural Genomics Consortium (University of Toronto, Toronto, ON).. Recombinant human phenylethanolamine- N -methyltransferase (hPNMT) and nicotinamide- N -methyltransferase (hNNMT) were purchased from Creative BioMart (Shirley, NY).. All reagents for the hPNMT assay were prepared in 50 mM Tris-HCl buffer (pH 8.5) unless stated otherwise, excluding PDAT, which was dissolved in dimethyl sulfoxide (DMSO).All reagents for the hPNMT assay were prepared in 50 mM Tris-HCl buffer (pH 8.5) unless stated otherwise, excluding PDAT, which was dissolved in dimethyl sulfoxide (DMSO).