Recombinant Protein A, His-tagged


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Recombinant Protein A, His-tagged

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Cat. No. : COP09
ProductOverview : Recombinant Staphylococcus Aureus ProteinA (Ala2-Cys293) contains 299 amino acids and a His tag at the N-terminus, wasexpressed in E. coli.
Description : Protein A is a 40-60kDa surface protein originally found in the cell wall of the bacteriaStaphylococcus aureus (SPA). SPA binds proteins from many of mammalianspecies, most notably IgGs, and helps inhibit phagocytic engulfment and actsas an immunological disguise viathis type of interaction, thus the bacteria will disrupts opsonization andphagocytosis. It binds with the Fc region of immunoglobulins throughinteraction with the heavy chain. It couples to a wide variety of reportermolecules including fluorescent dyes, enzyme markers, biotin, colloidal goldand radioactive iodine without affecting the antibody binding site.Recombinant Protein A was developed to increase the specificity of themolecule for IgG and is widely used both in research and bioprocessing.
Source : E.coli.
Form : Bulk protein in a 0.22 μm filtered solutionof PBS, pH 7.4 and delivered as liquid formulation or lyophilizedpowder.Normally 5-8% trehalose and mannitol are added as protectants beforelyophilization.
Molecularweight : 33.8kDa
Endotoxin : Less than 0.01 ngendotoxin/μg Recombinant Protein A.
Purity : >95%as determined by SDS-PAGE of 6 μg reduced recombinant proteins. All lots aregreater than 95% pure.
Storage : Store at -20℃in lyophilized state after receipt. For long term storage, upon reconstitutionRecombinant Protein A should be aliquot and store at -20℃ or -80℃. Avoid repeatedfreeze-thaw cycles.
Background :
IntroductionStaphylococcusaureus is a facultative anaerobic Gram-postive coccal bacterium. It isfrequently found as part of the normal skin flora on the skin and nasalpassages. S. aureus is the most commonspecies of staphylococci to cause Staph infections. S. aureus cancause a range of illnesses from minor skin infections,such as pimples, impetigo, boils (furuncles), cellulitis folliculitis, carbuncles, scaldedskin syndrome, and abscesses, to life-threateningdisease such as pneumonia, meningitis, osteomyelitis, endocarditis, toxic shock syndrome (TSS), bacteremia, and sepsis.
KeywordsImmunoglobulinG binding protein A; Staphylococcalprotein A;gG-binding protein A ; SPA; SpA; Protein A; RPA; Protein A
Structureof a domain of protein A as a three-helix bundle binding to the heavyvariable chain of a VH3 human Fableft.Minimized protein A bound to Fc fragment of Rituximab. :

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