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Recombinant Human General Transcription Factor IIB, His tagged

Cat. No.: GTF2B-1831H
Product Overview: Recombinant GTF2B protein was expressed in E.coli and purified by using conventional chromatography techniques.
Description: GTF2B, also known as transcription factor II B, is one of several general transcription factors that make up the RNA polymerase II preinitiation complex. It localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. The protein serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. It is involved in the selection of the transcription start site.
Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS EWRTFSNDKA TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK YQNRRTMSSS DRAMMNAFKE ITTMADRINL PRNIVDRTNN LFKQVYEQKS LKGRANDAIA SACLYIACRQ EGVPRTFKEI CAVSRISKKE IGRCFKLILK ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV ELDLVPGRSP ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF PTDFKFDTPV DKLPQL
Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl
Molecular Weight: 36.9 kDa (336aa)
Purity: > 90% by SDS - PAGE
Concentration: 0.5 mg/ml (determined by Bradford assay)
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways: Basal transcription factors; Gene Expression; HIV Infection; Transcription.
Gene Name: GTF2B general transcription factor IIB [ Homo sapiens ]
Synonyms: GTF2B; TF2B; TFIIB; general transcription factor IIB; RNA polymerase II transcription factor IIB; S300-II.
Gene ID: 2959
mRNA Refseq: NM_001514
Protein Refseq: NP_001505
MIM: 189963
UniProt ID: Q00403
Chromosome Location: 1p22-p21
Function: DNA binding; general RNA polymerase II transcription factor activity; metal ion binding; transcription factor binding; transcription regulator activity; translation initiation factor activity; zinc ion binding.

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