"Il4" Related Products

Recombinant Rat Interleukin 4

Cat. No.: Il4-96R
Product Overview: Recombinant Rat Interleukin-4 produced inE.Coliis a single, non-glycosylated polypeptide chain containing 140 amino acids and having a molecular mass of 16 kDa. The IL-4 is purified by proprietary chromatographic techniques.
Description: IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Source: Escherichia Coli.
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Gly-Cys-Asn.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Solubility: It is recommended to reconstitute the lyophilized I-TAC in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Specific Activity: The ED50 as determined by the dose-dependant prolifiration of Con A activated rat spleen cells is < 1.5 ng/ml, corresponding to a Specific Activity of 6.6 x 105 IU/mg.
Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard.
Gene Name: Il4 interleukin 4 [ Rattus norvegicus ]
Synonyms: Il4; interleukin 4; Il4e12
Gene ID: 287287
mRNA Refseq: NM_201270
Protein Refseq: NP_958427
UniProt ID: P20096
Chromosome Location: 10q22
Pathway: Allograft rejection; Autoimmune thyroid disease; Fc epsilon RI signaling pathway; Jak-STAT signaling pathway; T cell receptor signaling pathway
Function: cytokine activity; growth factor activity; interleukin-4 receptor binding; protein binding

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.