||Recombinant human AXL was expressed by baculovirus inSf9 insect cellsusing an N-terminal His tag. MW = 77 kDa.
||AXL is a member of the receptor tyrosine kinase family which has oncogenic potential and is implicated in human myeloid leukemia. AXL is a member of a complex signaling network that is involved in the control of cell proliferation and differentiation. Overexpression of AXL cDNA in NIH 3T3 cells induces neoplastic transformation of these cells with the concomitant appearance of a 140kDa AXL tyrosine-phosphorylated protein. Expression of AXL cDNA in the baculovirus system results in the expression of the appropriate recombinant protein that is recognized by antiphosphotyrosine antibodies, confirming that the AXL protein is tyrosine phosphorylated.
||Sf9 insect cells using baculovirus.
||140 U/mg. One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to Tyr substrate per minute at pH 7.4 and 30ºC. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 μM Na-orthovanadate, 0.5 mM ATP, 30 μg/ml Poly (Glu:Tyr)4:1 substrate, and 1 μg/ml recombinant AXL.
||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
||25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
||>6 months at –80°C.