|Cat. No. :
|Product Overview :
||Recombinant human EPHA2 was expressed by baculovirus inSf9 insect cellsusing an N-terminal His tag. MW=47kDa.
||EPHA2 is a member of the ephrin receptor subfamily of protein-tyrosine kinases that bind the ephrin-A ligand and have diverse cellular function. EPHA2 has been shown to be an oncoprotein of importance in a range of cancers. EPHA2 is overexpressed in several human cancer types and promotes malignancy through a mechanism involving RhoA-dependent destabilization of adherens junctions. EPHA2 overexpression induces a FAK-dependent increase in MMP-2 expression and invasiveness and this process that can be reversed by ligation of EPHA2.
||Sf9 insect cells.
|Specific Activity :
||170 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30ºC. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 μM Na-orthovanadate, 0.1 mM ATP, 30 μg/ml Poly (Glu:Tyr) 4:1 substrate, and 0.1 μg /ml recombinant EPHB2.
||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
|Formulated In :
||50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 50% glycerol and 3mM DTT.
||>12 months at –80°C.