|Cat. No. :
|Product Overview :
||Recombinant human ADAMTS-1 (residues 213 to 579 of full-length human ADAMTS-4) protein with His tag, was expressed in abaculovirussystem and purified from insect cells, 40 kDa.
||Aggrecanases belong to a family of ADAMTS proteases (ADAMTS = A disintegrin and metalloprotease with thrombospondin motifs). ADAMTS enzymes are comprised of an N-terminal prodomain, a catalytic domain, a disintegrin domain, one or more thrombospondin (TS) motifs, a cysteine-rich domain and a spacer domain of variable length. The prodomains are cleaved off intracellularly and the enzymes are processed further from their C-terminal ends after secretion. Aggrecanases are responsible for aggrecan degradation in osteoarthritic joints. ADAMTS-4 (aggrecanase-1) is a major aggrecanase in human osteoarthritic cartilage.5 ADAMTS-4 hydrolyzes also versican and brevican.
||Activity (mU/mg) is specified on the vial.
|Unit Definition :
||One milliunit of protease hydrolyzes 1 nanomole of aggrecan interglobular domain substrate per minute at pH 7.5 at 37°C.
||> 90% as estimated by SDS-PAGE.
||50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2, 0.05% Brij-35.
||Used for screening of ADAMTS-4 inhibitors and for continuous assay of ADAMTS-4 activity and for FRET-based assay.
||Store at -80°C. Avoid multiple thaw-freeze cycles.