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|Product Overview :
||Recombinant Rat Fibroblast Growth Factor 7 was expressed inE.Coli.MW=17 kDa.
||Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells.
||Purity > 90%. Purified by heparin affinity chromatography.
||The activity of rat FGF-7 at 1-10 ng/ml is determined by tritiated thymidine incorporation using cultured mouse keratinocytes. Maximum stimulation observed at 1 ng/ml. The optimal concentration of this product for use with other cell types must be determined by the end user.
||45102 binds to keratinocytes and most parenchymal epithelial cells; binds specific splice variant of FGF receptor expessed in epithelial cells (FGFr2IIIb).
||Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of IGF-II as a Reference Standard.
||This product is stable for at least one year at -20℃ to -70℃. Keep frozen until use. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.