|Cat. No. :
|Product Overview :
||Recombinant Human SEMA6B fussed with a Fc region of human IgG1 at the C-terminus via a peptide linker was expressed in a mouse myeloma cell line,NS0.
||This gene encodes a member of the semaphorin family, a group of proteins characterized by the presence of a conserved semaphorin (sema) domain. Whereas some semaphorins are transmembrane proteins, others are secreted. Semaphorins play a major role in axon guidance. The protein encoded by this gene may be involved in both peripheral and central nervous system development.
||> 95%, as determined by SDS-PAGE and visualized by silver stain.
|Molecular Mass :
||The mature recombinant human Semaphprin 6B/Fc chimera, generated by proteolytic removal of the signal peptide region, is a homodimeric protein. Based on N-terminal sequencing, the mature recombinant human Semaphorin 6B protein starts at Leu 26. The monomer of the recombinant human Semaphorin 6B has a calculated molecular mass of approximately 63.4 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 110 - 120 kDa protein in SDS-PAGE under reducing conditions.
||Measured by its ability to cause collapse of chick DRG growth cones. The ED50 for this effect is typically 2.5 - 5.0 μg/mL in the presence of 10 ng/mL of rhβ-NGF.
||< 1.0 EU per 1 μg of the enzyme as determined by the LAL method.
||Lyophilized from a 0.2 μm filtered solution in PBS.
||Samples are stable for up to six months at -70°C in a manual defrost freezer after the date of receipt. After receiving, this protein can be aliquoted and stored at above temperature for up to six months without a significant loss of activity. Avoid repeated freeze-thaw cycles.