||Recombinant Human Deoxynucleotidyltransferase, Terminal encoding 1-200 amino acids expressed inE.coli. The TDT is purified by proprietary chromatographic techniques.
||Terminal Deoxynucleotidyl Transferase (TdT). TdT is a DNA polymerase located in the cell nucleus which catalyses the polymerization of deoxynucleotides at the 3-hydroxyl ends of oligo or polydeoxynucleotide initiators and functions without a template. TdT is considered to be a highly specific marker for the diagnosis and classification of acute lymphoblastic lymphoma/leukemias. The determination of TdT expression is most valuable when it is different to differentiate histologically between lymphoblastic lymphoma and Burkitt‘s lymphoma.
||Sterile filtered liquid.
||TDT protein at 100μg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.
||Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months. Please avoid freeze-thaw cycles. Application And Suggested Dilutions: 1. ELISA 2. Inhibition Assays 3. Western Blotting Characterization: On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 50 kDa.