Recombinant Human Deoxynucleotidyltransferase, Terminal

• Cat.No.: DNTT-563H
• Product Overview: Recombinant Human Deoxynucleotidyltransferase, Terminal encoding 1-200 amino acids expressed inE.coli. The TDT is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 1-200 a.a.
• Description: Terminal Deoxynucleotidyl Transferase (TdT). TdT is a DNA polymerase located in the cell nucleus which catalyses the polymerization of deoxynucleotides at the 3-hydroxyl ends of oligo or polydeoxynucleotide initiators and functions without a template. TdT is considered to be a highly specific marker for the diagnosis and classification of acute lymphoblastic lymphoma/leukemias. The determination of TdT expression is most valuable when it is different to differentiate histologically between lymphoblastic lymphoma and Burkitt‘s lymphoma.
• Physical Appearance: Sterile filtered liquid.
• Formulation: TDT protein at 100μg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.
• Stability: Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months. Please avoid freeze-thaw cycles. Application And Suggested Dilutions: 1. ELISA 2. Inhibition Assays 3. Western Blotting Characterization: On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 50 kDa.

Gene Information

• Gene Name: DNTT deoxynucleotidyltransferase, terminal [ Homo sapiens ]
• Synonyms: DNTT; deoxynucleotidyltransferase, terminal; DNA nucleotidylexotransferase; nucleosidetriphosphate:DNA deoxynucleotidylexotransferase; terminal addition enzyme; terminal deoxynucleotidyltransferase; terminal deoxyribonucleotidyltransferase; terminal transferase; TDT; EC 2.7.7.31
• Gene ID: 1791
• mRNA Refseq: NM_001017520
• Protein Refseq: NP_001017520
• MIM: 187410
• UniProt ID: P04053
• Chromosome Location: 10q23-q24
• Pathway: Hematopoietic cell lineage; Non-homologous end-joining
• Function: DNA binding; DNA nucleotidylexotransferase activity; DNA-directed DNA polymerase activity; metal ion binding; transferase activity