"DNTT" Related Products


Recombinant Human Deoxynucleotidyltransferase, Terminal

Cat. No.: DNTT-563H
Product Overview: Recombinant Human Deoxynucleotidyltransferase, Terminal encoding 1-200 amino acids expressed inE.coli. The TDT is purified by proprietary chromatographic techniques.
Description: Terminal Deoxynucleotidyl Transferase (TdT). TdT is a DNA polymerase located in the cell nucleus which catalyses the polymerization of deoxynucleotides at the 3-hydroxyl ends of oligo or polydeoxynucleotide initiators and functions without a template. TdT is considered to be a highly specific marker for the diagnosis and classification of acute lymphoblastic lymphoma/leukemias. The determination of TdT expression is most valuable when it is different to differentiate histologically between lymphoblastic lymphoma and Burkitt‘s lymphoma.
Source: Escherichia Coli.
Physical Appearance: Sterile filtered liquid.
Formulation: TDT protein at 100μg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.
Stability: Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months. Please avoid freeze-thaw cycles. Application And Suggested Dilutions: 1. ELISA 2. Inhibition Assays 3. Western Blotting Characterization: On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 50 kDa.
Gene Name: DNTT deoxynucleotidyltransferase, terminal [ Homo sapiens ]
Synonyms: DNTT; deoxynucleotidyltransferase, terminal; DNA nucleotidylexotransferase; nucleosidetriphosphate:DNA deoxynucleotidylexotransferase; terminal addition enzyme; terminal deoxynucleotidyltransferase; terminal deoxyribonucleotidyltransferase; terminal transferase; TDT; EC
Gene ID: 1791
mRNA Refseq: NM_001017520
Protein Refseq: NP_001017520
MIM: 187410
UniProt ID: P04053
Chromosome Location: 10q23-q24
Pathway: Hematopoietic cell lineage; Non-homologous end-joining
Function: DNA binding; DNA nucleotidylexotransferase activity; DNA-directed DNA polymerase activity; metal ion binding; transferase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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