|Cat. No. :
|Product Overview :
||Recombinant Human Interleukin-3 produced inSaccharomyces cerevisiaeis a single, glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 18 kDa. As a result of glycosilation, the protein migrates at 19.5 kDa on SDS-PAGE.
||IL24 is a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. IL-10B encoded can induce apoptosis selectively in various cancer cells. Overexpression IL-24 leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14, and heat shock 27kDa protein 1 are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes.
|Physical Appearance :
||Sterile Filtered white lyophilized (freeze-dried) powder.
||Lyophilized from a 0.2nm filtered solution in PBS with BSA as a carrier.
||Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
||It is recommended to reconstitute the lyophilized Interleukin-24 in sterile 18Mω-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
|Biological Activity :
||Measured by its ability to bind to the cell receptor of Capan-1 cells line resulted in Stat-3 activation. The ED50 for this effect is typically 1.0 ng/mL.
||Lyophilized MDA7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MDA-7 Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.