Recombinant Human Interleukin 3 (Colony-Stimulating Factor, Multiple)

• Cat.No.: IL3-275H
• Product Overview: Recombinant Human Interleukin-3 produced inSaccharomycescerevisiae is a single, glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. The recombinant human IL-3 migrates as a broad band between 15 and 22 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation.

Specification

• Cat. No.: IL3-275H
• Description: IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
• Source: Saccharomyces cerevisiae.
• AA Sequence: A P M T Q T T P L K T S W V N C S N M I D E I I T H L K Q P P L P L L D F N N L N G E D Q D I L M E N N L R R P N L E A F N R A V K S L Q N A S A I E S I L K N L L P C L P L A T A A P T R H P I H I K D G D W N E F R R K L T F Y L K T L E N A Q A Q Q T T L S L A I F.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM Phosphate buffer pH-6.3.
• Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Solubility: It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18Mω-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED50 of IL-3 is typically 0.1- 0.4 ng/ml as measured in a cell proliferation assay using the human growth factor dependent TF-1 cell line.
• Stability: Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: IL3 interleukin 3 (colony-stimulating factor, multiple) [ Homo sapiens ]
• Synonyms: IL3; interleukin 3 (colony-stimulating factor, multiple); IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF; P-cell stimulating factor; hematopoietic growth factor; interleukin 3; mast-cell growth factor; multilineage-colony -stimulating factor; MULTI-CSF; Hematopoietic growth factor; P-cell-stimulating factor; Mast cell growth factor; Multipotential colony-stimulating factor
• Gene ID: 3562
• mRNA Refseq: NM_000588
• Protein Refseq: NP_000579
• MIM: 147740
• UniProt ID: P08700
• Chromosome Location: 5q31.1
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Asthma; Hematopoietic cell lineage; Fc epsilon RI signaling pathway
• Function: interleukin-3 receptor activity; cytokine activity; growth factor activity; protein binding