||Recombinant Human PLA2G4A is manufactured with N-terminal fusion HisTag. sPLA2-X His-Tagged Fusion Protein, is 15.5 kDa containing 123 amino acid residues of the human secreted phospholipase A2. It is expressed inEscherichia Coli.
||Phospholipase A2 (PLA2) catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid (AA), a precursor of eicosanoids including prostaglandins and leukotrienes. The same reaction also produces lysophosholipids, which represent another class of lipid mediators. The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of low molecular weight, Ca2+requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense.
|Amino Acid Sequence:
||MRGSHHHHHHGMASHMGILELAGTVGCVGPRTPIAYMKYGCFCGLGGHGQ RDAIDWCCHGHDCCYTRAEEAGCSPKTERYSWQCVNQSVLCGPAENKCQE LLCKCDQEIANCLAQTEYNLKYLFYPQFLCEPDSPKCD.
||Sterile Filtered lyophilized (freeze-dried) powder.
||Greater than 95% as determined by SDS PAGE.
||Sterile filtered and lyophilized from 0.5 mg/ml in 0.01M Tris buffer pH 7.2.
||Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C.