Recombinant Human UCHL1, GST-tagged UCHL1-49H

Recombinant Human UCHL1, GST-tagged


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Recombinant Human UCHL1, GST-tagged

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Cat. No. : UCHL1-49H
Product Overview : Recombinant human full length PGP9.5 protein with GST-tag was expressed inE.coli.
Description : Protein gene product (PGP9.5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. The protein which has a molecular weight of 27kDa was first identified by high resolution two dimensional PAGE. Standard immunohistochemical techniques have demonstrated the presence of PGP9.5 in neurons and nerve fibres at all levels of the central and peripheral nervous system, in many neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum.
Source : E.coli.
Application And Suggested Dilutions : ELISA Inhibition Assays Western Blotting.
Characterization : On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 36kDa.
Presentation : 100μl purified human PGP9.5 protein at 100μg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol.
Storage & Stability : Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months.
PDB rendering based on 2etl. :

Gene Name : UCHL1 ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) [ Homo sapiens ]
Synonyms : UCHL1; PARK5; PGP9.5; Uch-L1; UCH-L1; EC, EC 6; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; ubiquitin C-terminal esterase L1; ubiquitin carboxyl-terminal esterase L1; ubiquitin thiolesterase L1; Ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Ubiquitin carboxy-terminal hydrolase L1; Ubiquitin carboxy-terminal hydrolase L1 (UCH-L1); EC; ubiquitin thiolesterase; ubiquitin carboxy-terminal esterase; isopeptidase; isopeptidase T; ubiquitin C-terminal hydrolase; ubiquitin carboxy-terminal hydrolase; ubiquitin-C-terminal-thiolester hydrolase; ubiquitin-C-terminal-thioester hydrolase
Gene ID : 7345
mRNA Refseq : NM_004181
Protein Refseq : NP_004172
MIM : 191342
UniProt ID : P09936
Chromosome Location : 4p14
Pathway : Neurodegenerative Disorders; Parkinson"s disease
Function : cysteine-type endopeptidase activity; ligase activity; omega peptidase activity; peptidase activity; protein binding; ubiquitin binding; ubiquitin thiolesterase activity

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