Active Recombinant Human UCHL1 protein, His-tagged

• Cat.No.: UCHL1-3091H
• Product Overview: Active Recombinant Human UCHL1 protein(NP_004172.2) (Gln 2-Ala 223) was expressed in E.coli, with a polyhistide tag at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-223 aa
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 20% glycerol, 1mM DTT, pH 8.0.
• Bio-activity: Measured by the hydrolysis of UbiquitinAMC. The specific activity is >140 pmoles/min/μg.
• Molecular Mass: The recombinant human UCHL1 consisting of 229 amino acids and migrates as an 25.6 kDa band in SDS-PAGE under reducing conditions as predicted.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: UCHL1 ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) [ Homo sapiens ]
• Official Symbol: UCHL1
• Synonyms: UCHL1; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); PARK5; ubiquitin carboxyl-terminal hydrolase isozyme L1; PGP9.5; Uch L1; ubiquitin thioesterase L1; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase; PGP95; Uch-L1; PGP 9.5
• Gene ID: 7345
• mRNA Refseq: NM_004181
• Protein Refseq: NP_004172
• MIM: 191342
• UniProt ID: P09936