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Recombinant Mouse ADP-ribosyltransferase 2b, His-tagged

Cat.No. : Art2b-2068M
Product Overview : Recombinant MouseART2.2 is produced inE. coliwith aC-terminus His-tag.
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Cat. No. : Art2b-2068M
Description : Extracellular NAD induces the ATP-independent activation of the ionotropic P2X(7) purinergic receptor (P2X(7)R) in murine T lymphocytes via a novel covalent pathway involving ADP-ribosylation of arginine residues on the P2X(7)R ectodomain. This modification is catalyzed by ART2.2, a GPI-anchored ADP-ribosyltransferase (ART) that is constitutively expressed in murine T cells. We previously reported that ART2.1, a related ecto-ART, is up-regulated in inflammatory murine macrophages that constitutively express P2X(7)R. Thus, we tested the hypothesis that extracellular NAD acts via ART2.1 to regulate P2X(7)R function in murine macrophages. Coexpression of the cloned murine P2X(7)R with ART2.1 or ART2.2 in HEK293 cells verified that P2X(7)R is an equivalent substrate for ADP-ribosylation by either ART2.1 or ART2.2. However, in contrast with T cells, the stimulation of macrophages or HEK293 cells with NAD alone did not activate the P2X(7)R. Rather, NAD potentiated ATP-dependent P2X(7)R activation as indicated by a left shift in the ATP dose-response relationship.
Source : E. coli.
Concentration : 0.5mg/ml.
Formulation : Liquid. In PBS containing 50% glycerol.
Purity : ≥98%.
Storage : -80°C. Avoid freeze/thaw cycles.
Gene Name : Art2b ADP-ribosyltransferase 2b [ Mus musculus ]
Synonyms : Art2b; ADP-ribosyltransferase 2b; T-cell NAD(P)(+)--arginine ADP-ribosyltransferase 2; T-cell differentiation marker Rt6 homolog 2; T-cell ecto-ADP-ribosyltransferase 2; T-cell mono(ADP-ribosyl)transferase 2; mono(ADP-ribosyl)transferase 2B; Art; Rt6; Rt-6; Ly92b; Rt6-2; ART2.2; Art2b
Gene ID : 11872
mRNA Refseq : NM_019915
Protein Refseq : NP_064299
UniProt ID : O35975
Chromosome Location : 7 E3; 7 49.0 cM
Function : NAD(P)+-protein-arginine ADP-ribosyltransferase activity; transferase activity; transferase activity, transferring glycosyl groups

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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Are there any known diseases or disorders associated with mutations in the ART2B gene? 07/26/2022

Mutations in the ART2B gene have not been directly linked to specific diseases or disorders in humans so far.

What are some known substrates of ART2B? 05/21/2021

ART2B ADP-ribosylates specific arginine residues on target proteins, including P2X purinergic receptors and certain immune cell markers.

Is the activity of ART2B influenced by any cofactors or regulators? 05/07/2021

ART2B activity is dependent on the presence of NAD+ as a substrate and can also be influenced by extracellular ATP levels and calcium concentrations.

Are there any therapeutic applications targeting ART2B? 02/22/2019

Currently, there are no specific therapeutics targeting ART2B for direct modulation. However, research on ADP-ribosylation and its associated pathways may have therapeutic implications in the future.

Does ART2B have any non-enzymatic functions or interactions with other proteins? 01/17/2018

Apart from its enzymatic role in ADP-ribosylation, ART2B does not have any known non-enzymatic functions or specific protein interactions.

Does ART2B activity change under pathological conditions? 10/22/2017

The dysregulation of ADP-ribosylation, including ART2B activity, has been implicated in various diseases. However, the specific role of ART2B in these conditions requires further investigation.

How does ART2B catalyze ADP-ribosylation? 08/28/2017

ART2B transfers ADP-ribose from NAD+ to specific target proteins, modifying their structure and function.

Customer Reviews (3)

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Reviews
11/01/2020

    Its impeccable purity and consistency have consistently delivered accurate and robust results, enabling me to make significant strides in my research.

    08/29/2017

      I confidently recommend the ART2B protein to any researcher seeking a superior protein that not only fulfills their experimental needs but is also backed by unparalleled support from the manufacturer.

      07/28/2017

        The Aldehyde Protein exhibits an exceptional level of quality that surpasses my experimental requirements.

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