Recombinant Mouse ADP-ribosyltransferase 2b, His-tagged
Cat.No. : | Art2b-2068M |
Product Overview : | Recombinant MouseART2.2 is produced inE. coliwith aC-terminus His-tag. |
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- Gene Information
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Cat. No. : | Art2b-2068M |
Description : | Extracellular NAD induces the ATP-independent activation of the ionotropic P2X(7) purinergic receptor (P2X(7)R) in murine T lymphocytes via a novel covalent pathway involving ADP-ribosylation of arginine residues on the P2X(7)R ectodomain. This modification is catalyzed by ART2.2, a GPI-anchored ADP-ribosyltransferase (ART) that is constitutively expressed in murine T cells. We previously reported that ART2.1, a related ecto-ART, is up-regulated in inflammatory murine macrophages that constitutively express P2X(7)R. Thus, we tested the hypothesis that extracellular NAD acts via ART2.1 to regulate P2X(7)R function in murine macrophages. Coexpression of the cloned murine P2X(7)R with ART2.1 or ART2.2 in HEK293 cells verified that P2X(7)R is an equivalent substrate for ADP-ribosylation by either ART2.1 or ART2.2. However, in contrast with T cells, the stimulation of macrophages or HEK293 cells with NAD alone did not activate the P2X(7)R. Rather, NAD potentiated ATP-dependent P2X(7)R activation as indicated by a left shift in the ATP dose-response relationship. |
Source : | E. coli. |
Concentration : | 0.5mg/ml. |
Formulation : | Liquid. In PBS containing 50% glycerol. |
Purity : | ≥98%. |
Storage : | -80°C. Avoid freeze/thaw cycles. |
Gene Name : | Art2b ADP-ribosyltransferase 2b [ Mus musculus ] |
Synonyms : | Art2b; ADP-ribosyltransferase 2b; T-cell NAD(P)(+)--arginine ADP-ribosyltransferase 2; T-cell differentiation marker Rt6 homolog 2; T-cell ecto-ADP-ribosyltransferase 2; T-cell mono(ADP-ribosyl)transferase 2; mono(ADP-ribosyl)transferase 2B; Art; Rt6; Rt-6; Ly92b; Rt6-2; ART2.2; Art2b |
Gene ID : | 11872 |
mRNA Refseq : | NM_019915 |
Protein Refseq : | NP_064299 |
UniProt ID : | O35975 |
Chromosome Location : | 7 E3; 7 49.0 cM |
Function : | NAD(P)+-protein-arginine ADP-ribosyltransferase activity; transferase activity; transferase activity, transferring glycosyl groups |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionMutations in the ART2B gene have not been directly linked to specific diseases or disorders in humans so far.
ART2B ADP-ribosylates specific arginine residues on target proteins, including P2X purinergic receptors and certain immune cell markers.
ART2B activity is dependent on the presence of NAD+ as a substrate and can also be influenced by extracellular ATP levels and calcium concentrations.
Currently, there are no specific therapeutics targeting ART2B for direct modulation. However, research on ADP-ribosylation and its associated pathways may have therapeutic implications in the future.
Apart from its enzymatic role in ADP-ribosylation, ART2B does not have any known non-enzymatic functions or specific protein interactions.
The dysregulation of ADP-ribosylation, including ART2B activity, has been implicated in various diseases. However, the specific role of ART2B in these conditions requires further investigation.
ART2B transfers ADP-ribose from NAD+ to specific target proteins, modifying their structure and function.
Customer Reviews (3)
Write a reviewIts impeccable purity and consistency have consistently delivered accurate and robust results, enabling me to make significant strides in my research.
I confidently recommend the ART2B protein to any researcher seeking a superior protein that not only fulfills their experimental needs but is also backed by unparalleled support from the manufacturer.
The Aldehyde Protein exhibits an exceptional level of quality that surpasses my experimental requirements.
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