|Cat. No. :
|Product Overview :
||Recombinant Rat Prkg2 was produced inSf9cells. MW = 86kDa; monomer.
||PKG are serine/threonine kinases that are present in a variety of eukaryotes ranging from the unicellular organism Paramecium to humans. Two PKG genes, coding for PKG type I (PKG-I) and type II (PKG-II), have been identified in mammals. The N-terminus of PKG-I is encoded by two alternatively spliced exons that specify for the PKG-Iα and PKG-Iβ isoforms. PKG-Iβ is activated at ~10-fold higher cGMP concentrations than PKG-Iα. The PKG-I and PKG-II are homodimers of two identical subunits (~75 kDa and ~85 kDa, respectively) and share common structural features.
||Liquid. In 20mM TRIS, pH 7.4, containing 1mM EDTA, 5mM β-mercaptoethanol, 100mM NaCl, 10U/ml trasylol, 0.015% NP40 and 50% glycerol.
|Specific Activity :
||~0.5-2.0U/mg protein. 5-fold activation by cGMP (5µM). One unit corresponds to 1µmol/mg/min using VASPtide (RRKVSKQE) as substrate, under the following optimal conditions: 10mM HEPES (pH 7.4), 5mM MgCl2, 1mM DTE, 0.2mM EDTA.
||The enzyme is relatively unstable. To prevent loss of activity when performing experiments, keep the stock vial of the enzyme on ice (0-4°C) at all times. The enzyme loses activity during a single freeze/thaw cycle. To avoid adherence of the product to plastic, the use of siliconized vials and tips is recommended. After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.