"CASP1" Related Products


Recombinant Human CASP1

Cat. No. : CASP1-596H
Description : Caspase-1 is the prototypical member of the caspase family of cysteine proteases. Caspase-1 exists in cells as an inactive 45kDa pro-enzyme. The pro-enzyme is matured by proteolysis to yield large (20kDa) and small (10kDa) subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of caspase-1 activation is complex and poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive pro-enzyme form.
Sourse/Host : E. coli.
Formulation : Lyophilized.
Reconstitution : Reconstitute to 1U/µl with PBS containing 15% glycerol.
Specific Activity : ~20'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate YVAD-pNA (pNA:p-nitroanaline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
Application : Useful in studying enzyme regulation and inhibitors, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
Storage : For long term storage, -80°C.
Pathways : Amyotrophic lateral sclerosis (ALS); Cytosolic DNA-sensing pathway; NOD-like receptor signaling pathway
PDB rendering based on 1bmq. :

Gene Name : CASP1 caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) [ Homo sapiens ]
Synonyms : caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); ICE; P45; IL1BC; CASP1; caspase-1; CASP-1; IL-1BC; IL1B-convertase; CASP1 nirs variant 1; IL-1 beta-converting enzyme; interleukin 1-beta convertase; interleukin-1 beta convertase; interleukin 1-B converting enzyme; interleukin-1 beta-converting enzyme; caspase 1, apoptosis-related cysteine protease (interleukin 1, beta, convertase); p45; EC
Gene ID : 834
mRNA Refseq : NM_001223
Protein Refseq : NP_001214
MIM : 147678
UniProt ID : P29466
Chromosome Location : 11q23
Function : cysteine-type endopeptidase activity; caspase activator activity; peptidase activity; protein binding

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