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Active Recombinant Human GSK3B protein, His-tagged

Cat.No. : GSK3B-616H
Product Overview : Recombinant Human GSK3B protein(NP_002084.2)(Met1-Thr433), fused with His tag, was expressed in Insect Cells.
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Source : Insect Cells
Species : Human
Tag : His
Form : Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 25% glycerol, 0.5mM PMSF, 0.5mM EDTAPlease contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity : 1. The specific activity was determined to be 45 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate.2. Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1, EC50 of biotinylated human HG3C-CTNNB1 is 0.15-0.35 μg/ml.
Molecular Mass : The recombinant human GSK3B consists of 452 amino acids and predicts a molecular mass of 50.4 kDa. The apparent molecular mass of rhGSK3B is approximately 44-48 kDa in SDS-PAGE under reducing conditions.
Protein Length : Met1-Thr433
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 90 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : GSK3B glycogen synthase kinase 3 beta [ Homo sapiens ]
Official Symbol : GSK3B
Synonyms : GSK3B; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK-3 beta; GSK3beta isoform; serine/threonine-protein kinase GSK3B;
Gene ID : 2932
mRNA Refseq : NM_001146156
Protein Refseq : NP_001139628
MIM : 605004
UniProt ID : P49841

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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