Active Recombinant Human S100A8, His tagged

• Cat.No.: S100A8-2216H
• Product Overview: Recombinant Human S100A8 (NP_002955.2) (Met 1-Glu 93), fused with a polyhistidine tag at the C-terminus, was produced in Baculovirus-Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: Met 1-Glu 93
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: Measured by its ability to bind recombinant human S100A9 in a functional ELISA.
• Molecular Mass: The recombinant human S100A8 consists of 103 amino acids and predicts a molecular mass of 12.2 kDa. It migrates as an approximately 14.6 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  Combining patient proteomics and in vitro cardiomyocyte phenotype testing to identify potential mediators of heart failure with preserved ejection fraction. (2016)
  Myeloid-related protein 8 induces self-tolerance and cross-tolerance to bacterial infection via TLR4-and TLR2-mediated signal pathways. (2015)

Gene Information

• Gene Name: S100A8 S100 calcium binding protein A8 [ Homo sapiens ]
• Official Symbol: S100A8
• Synonyms: S100A8; S100 calcium binding protein A8; CAGA, CFAG, S100 calcium binding protein A8 (calgranulin A) , S100 calcium binding protein A8 (calgranulin A); protein S100-A8; 60B8AG; CGLA; MRP8; P8; MRP-8; calgranulin A; calgranulin-A; cystic fibrosis antigen; calprotectin L1L subunit; urinary stone protein band A; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; S100 calcium-binding protein A8 (calgranulin A); MIF; NIF; CAGA; CFAG; L1Ag; CP-10; MA387
• Gene ID: 6279
• mRNA Refseq: NM_002964
• Protein Refseq: NP_002955
• MIM: 123885
• UniProt ID: P05109