| Cat. No. : |
AKR7A2-1934H |
| Description : |
Aldo-keto reductases, such as AKR7A2, are involved in the detoxification of aldehydes and ketones. This protein can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the nonbinding AFB1 dialcohol. It may be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen. Also, it has been proposed previously to catalyze the NADPH-dependent reduction of succinic semialdehyde (SSA) to gamma-Hydroxybutyrate in human brain. |
| Source : |
E.coli. |
| Purity : |
> 90% by SDS – PAGE. |
| Physical Appearance : |
Liquid. |
| Formulation : |
In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 20% glycerol. |
| Concentration : |
0.5 mg/ml (determined by Bradford assay). |
| Sequences Of Amino Acids : |
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM LSAASR VVSR AAVHCALRSP PPEARALAMS RPPPPRVASV LGT MEMG RRMD APASAAAVR AFLERGHTEL DTAFMYSDGQ SETILGGLGL GLGG GDC RVK IATKANPWDG KSLKPDSVRS QLETSLKRLQ CPQVDLF YLHAPD HGTPVEE TLHACQRLHQ EGKFVELGLS NYASWEVAEI CTLCKSNGWI LPTVYQGMYN ATTRQVETEL FPCLRHFGLR FYAYNPLAGGLLTGKYKYED KDGKQPVGRF FGNSWAETYR NRFWKEHHFE AIALVEKALQ AAYGASAPSV TSAALRWMYH HSQLQGAHGD AVILGMSSLEQLEQNLAATE EGPLEPAVVD AFNQAWHLVA HECPNYFR. |
| Biological activity : |
Specific activity: approximately 0.25-0.3 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25℃. Specific activity was expressed as units/mg protein. |
| Activity Assay : |
1.Prepare a 1.mll reaction mix into a suitable container: the final concentrations are 0.1M sodium phosphate (pH7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH.2.Add 50ul of recombinant AKR7A2 solution with various concentrations (2.5ug, 5ug, 10ug) in 750ul reaction buffer. 3.Mix by inversion and Incubate at 25℃ for 2.5 minutes.4.Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion.5.Record the increase in A340nm for 3 minutes. |
| Storage : |
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
