||Recombinant human GOPC protein fused to his-tag was expressed inE.coliand purified by using conventional chromatography techniques. 21.5 kDa (198aa).
||GOPC, also known as PIST, is a PDZ domain-containing Golgi protein. This protein functions as a homooligomer that interacts with a variety of proteins and plays a role in intracellular protein trafficking and degradation. Additionally, GOPC is thought to regulate ionic currents via membrane channel modification and may also play a role in autophagy. Chromosomal aberrations in the gene encoding GOPC are found in glioblastoma multiform (GBM), a common and aggressive form of brain tumor, suggesting a role for mutated PIST in carcinogenesis.
||> 90% by SDS – PAGE.
||In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 1mM DTT.
||1 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MIRKVLLLKE DHEGLGISIT GGK EHGVPIL ISEIHPGQPA DRCGGLHVGD AILAVNGVNL RDTKHKEAVT ILSQ QRGEIE FEVVYVAPEV DSDDENVEYE DESGHRYRLY LDEL EGGGNP GASCKDTSGE IKVLQGFNKK AVTDTHENGD LGTASETPLD DGASKLDDLH TLYHKKSY.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.