Active Recombinant Human PREP Protein, His-tagged

• Cat.No.: PREP-2376H
• Product Overview: Recombinant full length human Prolyl oligopeptidase (POP)/Prolyl endopeptidase (PREP) protein with N-terminal His-tag was expressed in an E. coli expression system.

Specification

• Description: The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Aqueous buffer solution
• Bio-activity: ≥ 30 pmol/min/µg.
• Molecular Mass: 81.6 kDa
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Usage: Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween 20, 20 µM DPP substrate 1 (A-P-AMC), 30 min at room temperature.
• Notes: OUR PRODUCTS ARE FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.
• Stability: At least 6 months at –80 centigrade. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
• Storage Buffer: 45 mM Tris-HCL, pH 8.0, 124 mM NaCL, 2.4 mM KCL, 3 mM DTT, 10% Glycerol and variable amounts of Imidazole.
• Warning: Avoid freeze/thaw cycles

Gene Information

• Gene Name: PREP prolyl endopeptidase [ Homo sapiens (human) ]
• Official Symbol: PREP
• Synonyms: PREP; prolyl endopeptidase; PE; PEP; prolyl endopeptidase; dJ355L5.1 (prolyl endopeptidase); post-proline cleaving enzyme; prolyl oligopeptidase; EC 3.4.21.26
• Gene ID: 5550
• mRNA Refseq: NM_002726
• Protein Refseq: NP_002717
• MIM: 600400
• UniProt ID: P48147