||Recombinant Human full lengthERCC1 (aa 448-568) is expressed inE.coli. MW=61kDa.
||The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3" of the DNA lesion while the ERCC1-XPF complex makes the 5" incision. Elevated levels of ERCC1 have also been reported in Cisplatin-resistant cells.
||100μl purified human MET protein at 100μg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol without BSA and Sodium Azide.
||ELISA, Inhibition Assays, Western Blotting.
||On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 61kDa including GST.
||Store vial at -20℃ to -80℃. When stored at the recommended temperature, this protein is stable for 12 months.