Recombinant Human PIK3CB/PIK3R1, FLAG-tagged

• Cat.No.: PIK3CB/PIK3R1-632H
• Product Overview: Recombinant human p110β and untagged p85α complex of N-terminal Flag-tagged were coexpressed in aBaculovirus infected Sf9 cellexpression system. p110β, MW=124 kDa, p85α, MW=83.7 kDa.

Specification

• Cat. No.: PIK3CB/PIK3R1-632H
• Description: The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110β catalytic subunit (PIK3CB) plays a role in regulating the formation and stability of alpha-2B-beta-3 integrin adhesion bonds, which are necessary for shear force-induced platelet activation. In animal model of prostate tumor formation induced by the tumor suppressor PTEN loss, ablation of p110β impedes tumorigenesis with a concomitant diminution of AKT phosphorylation.
• Source: Sf9 cells.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, and 3 mM DTT.
• Specific Activity: 100 pmole/min/ug. A unit of 110β/p85α kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3,4,5-Trisphosphate/min at 30°C. A 25μl kinase reaction is conducted in a buffer containing 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 5 mM MgCl2, 100 μM ATP, 10μg mix of PS and Phosphoinositol-4,5-Bisphosphate, and 50 ng enzyme for 15 minutes at 30°C. Conversion of ATP to ADP is detected using PI3K ADP-Glo® Luminescent Kinase Assay (Promega Corporation, Madison, WI).
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Stability: >6 months at –80°C.
• Pathways: Acute myeloid leukemia; Apoptosis; B cell receptor signaling pathway; Chemokine signaling pathway; Chronic myeloid leukemia; Colorectal cancer; Endometrial cancer; ErbB signaling pathway; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Focal adhesion; Glioma; Inositol phosphate metabolism; Insulin signaling pathway; Jak-STAT signaling pathway; Leukocyte transendothelial migration; Melanoma; Natural killer cell mediated cytotoxicity; Neurotrophin signaling pathway; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Phosphatidylinositol signaling; Progesterone-mediated oocyte maturation; Prostate cancer; Regulation of actin cytoskeleton; Renal cell carcinoma; Small cell lung cancer; T cell receptor signaling pathway; Toll-like receptor signaling pathway; Type II diabetes mellitus; VEGF signaling pathway; mTOR signaling pathway

Gene Information

• Gene Name: PIK3CB phosphoinositide-3-kinase, catalytic, beta polypeptide [ Homo sapiens ],PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha) [ Homo sapiens ]
• Synonyms: PIK3CB; phosphoinositide-3-kinase, catalytic, beta polypeptide; PI3K; PIK3C1; P110BETA; PI3KBETA; MGC133043; DKFZp779K1237; PtdIns-3-kinase p110; PI3-kinase p110 subunit beta; Phosphatidylinositol-4, 5-bisphosphate 3-kinase; catalytic subunit beta isoform; EC 2.7.1.153; PI3-kinase p110 subunit beta; PtdIns-3-kinase p110; PI3Kbeta; PIK3R1; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); p85; GRB1; p85-ALPHA
• Gene ID: 5291,5295
• mRNA Refseq: NM_006219,NM_181504
• Protein Refseq: NP_006210,NP_852556
• MIM: 602925,171833
• UniProt ID: P42338, P27986
• Chromosome Location: 3q21, 5q13.1
• Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; inositol or phosphatidylinositol kinase activity; insulin receptor substrate binding; nucleotide binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphotransferase activity, alcohol group as acceptor; transferase activity; ErbB-3 class receptor binding