Recombinant Human CHEK2, His-tagged

• Cat.No.: CHEK2-635H
• Product Overview: Recombinant Human full length check point kinase 2 with N-terminal His-tag was expressed in a Baculovirus infectedSf9 cellexpression system. MW=91kDa.

Specification

• Cat. No.: CHEK2-635H
• Description: CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage; the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner. Expression of wild-type Chk2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2 mutant abrogated both phosphorylation of p53 on Ser-20 and p53 stabilization.
• Source: Sf9 cells.
• Formulated In: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 40% glycerol and 3mM DTT.
• Specific Activity: 6.9 pmoles/min/μg. Assay conditions: CHEK2 was incubated with a substrate for 1h at RT in 1Xkinase buffer supplemented with ATP. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Stability: >12 months at –80°C.
• Pathways: Cell cycle; p53 signaling pathway; Cell Cycle Checkpoints

Gene Information

• Gene Name: CHEK2 CHK2 checkpoint homolog (S. pombe) [ Homo sapiens ]
• Synonyms: CHEK2; CHK2 checkpoint homolog (S. pombe); CDS1; CHK2; LFS2; RAD53; HuCds1; PP1425; CHK2 (checkpoint, S.pombe) homolog; Serine/threonine-protein kinase Chk2; EC2.7.11.1; Cds1
• Gene ID: 11200
• mRNA Refseq: NM_001005735
• Protein Refseq: NP_001005735
• MIM: 604373
• UniProt ID: O96017
• Chromosome Location: 22q12.1
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity; magnesium ion binding; protein binding