Recombinant Human CHEK2, His-tagged
Cat.No. : | CHEK2-635H |
Product Overview : | Recombinant Human full length check point kinase 2 with N-terminal His-tag was expressed in a Baculovirus infectedSf9 cellexpression system. MW=91kDa. |
- Specification
- Gene Information
- Related Products
Cat. No. : | CHEK2-635H |
Description : | CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage; the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner. Expression of wild-type Chk2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2 mutant abrogated both phosphorylation of p53 on Ser-20 and p53 stabilization. |
Source : | Sf9 cells. |
Formulated In : | 50mM Tris-HCl, pH 7.5, 150mM NaCl, 40% glycerol and 3mM DTT. |
Specific Activity : | 6.9 pmoles/min/μg. Assay conditions: CHEK2 was incubated with a substrate for 1h at RT in 1Xkinase buffer supplemented with ATP. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT. |
Application : | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Stability : | >12 months at –80°C. |
Pathways : | Cell cycle; p53 signaling pathway; Cell Cycle Checkpoints |
Gene Name : | CHEK2 CHK2 checkpoint homolog (S. pombe) [ Homo sapiens ] |
Synonyms : | CHEK2; CHK2 checkpoint homolog (S. pombe); CDS1; CHK2; LFS2; RAD53; HuCds1; PP1425; CHK2 (checkpoint, S.pombe) homolog; Serine/threonine-protein kinase Chk2; EC2.7.11.1; Cds1 |
Gene ID : | 11200 |
mRNA Refseq : | NM_001005735 |
Protein Refseq : | NP_001005735 |
MIM : | 604373 |
UniProt ID : | O96017 |
Chromosome Location : | 22q12.1 |
Function : | ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity; magnesium ion binding; protein binding |
Products Types
◆ Recombinant Protein | ||
CHEK2-596H | Recombinant Human CHEK2 Protein, His (Fc)-Avi-tagged | +Inquiry |
CHEK2-09H | Recombinant Active Human CHEK2 Protein, His-tagged | +Inquiry |
CHEK2-1188H | Recombinant Human CHEK2 Protein (S2-L543), Tag Free | +Inquiry |
CHEK2-3214H | Recombinant Human CHEK2 Protein, MYC/DDK-tagged | +Inquiry |
CHEK2-1189H | Recombinant Human CHEK2 Protein (S2-L543), GST tagged | +Inquiry |
◆ Lysates | ||
CHEK2-541MCL | Recombinant Mouse CHEK2 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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