||Recombinant Human full length check point kinase 2 with N-terminal His-tag was expressed in a Baculovirus infectedSf9 cellexpression system. MW=91kDa.
||CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage; the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner. Expression of wild-type Chk2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2 mutant abrogated both phosphorylation of p53 on Ser-20 and p53 stabilization.
||50mM Tris-HCl, pH 7.5, 150mM NaCl, 40% glycerol and 3mM DTT.
||6.9 pmoles/min/μg. Assay conditions: CHEK2 was incubated with a substrate for 1h at RT in 1Xkinase buffer supplemented with ATP. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT.
||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
||>12 months at –80°C.
||Cell cycle; p53 signaling pathway; Cell Cycle Checkpoints