Recombinant Mouse Chek2 Protein, His/GST-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Chek2-5172MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Mouse Chek2 (Q9Z265) (Mey 1-Leu 546), fused with the N-terminal polyhistidine-tagged GST tag, was produced in Baculovirus-Insect cells.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 783
• Form: Lyophilized
• Molecular Mass: The secreted recombinant mouse CHEK2/GST chimera consists of 783 amino acids and has a calculated molecular mass of 89 kDa. The recombinant protein migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Conjugation: Alexa Fluor 555

Gene Information

• Gene Name: Chek2 checkpoint kinase 2 [ Mus musculus ]
• Official Symbol: Chek2
• Gene ID: 50883
• mRNA Refseq: NM_016681
• Protein Refseq: NP_057890