| Source : |
HEK293 |
| Species : |
Rhesus |
| Tag : |
C-His |
| Protein length : |
Met1-His266,256Asn/Gln |
| Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 2A6 mouse embryonal fibroblast cells. The ED50 for this effect is typically 0.5-3 μg/ml in the presence of 10 ng/mL of Wnt3a. |
| Molecular Mass : |
The recombinant rhesus DKK1 comprises 246 amino acids with a predicted molecular mass of 27.2 kDa. As a result of glycosylation, the apparent molecular mass of rhesus DKK1 is approximately 38-43 kDa in SDS-PAGE under reducing conditions. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : |
> 95 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
