Recombinant Mouse SerpinD1 protein(Met1-Ser478), His-tagged

• Cat.No.: Serpind1-2310M
• Product Overview: Recombinant Mouse SerpinD1(NP_032249.3) (Met1-Ser 478) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1- a.a.
• Form: Lyophilized from sterile 25mM HEPES, 150mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit thombin (Sigma, T4648) cleavage of fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is < 1.2 nM.
• Molecular Mass: The recombinant mouse SerpinD1 consists of 466 amino acids and predicts a molecular mass of 53.5 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rmSerpinD1 is approximately 65-70 kDa as a result of glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Serpind1 serine (or cysteine) peptidase inhibitor, clade D, member 1 [ Mus musculus ]
• Official Symbol: SerpinD1
• Synonyms: SERPIND1; serine (or cysteine) peptidase inhibitor, clade D, member 1; heparin cofactor 2; HC-II; serpin D1; heparin cofactor II; protease inhibitor leuserpin-2; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1; HCII; Hcf2; AA985900; AI303446; MGC107662
• Gene ID: 15160
• mRNA Refseq: NM_008223
• Protein Refseq: NP_032249