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Recombinant Human Aurora Kinase B, GST-His

Cat. No. : AURKB-684H
Product Overview : Recombinant human Aurora B kinase consisting of mino acids 2-344 was fused at the N-terminus to a GST-His Tag sequence. It was expressed inS. frugiperdainsect cells using a baculovirus expression system.MW = 70000 Da.
Description : Aurora B is a mitotic kinase that phosphorylates histone H3 and CenpA. Upregulation of Aurora B is reported in pancreatic and prostate cancers. The active kinase is useful in the study of Aurora B regulation and for inhibitor screening.
Source/Host : S. frugiperda.
Form : Liquid. In 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% Tween®-20 detergent, pH 8.0. Please refer to vial label for lot-specific concentration.
Specific Activity : ≥12 units/µg protein.
Purity : ≥70% by SDS-PAGE.
Unit Definition : One unit is defined as the amount of enzyme that is required to phosphorylate 1 pmol Ser/Thr 1 peptide substrate per min at 30°C, pH 7.4.
Toxicity : Standard Handling (MSDS available upon request).
Storage : ≤ -70°C. Avoid freeze/thaw Following intitial thaw, aliquot and freeze (-70°C).
Gene Name : AURKB aurora kinase B [ Homo sapiens ]
Synonyms : AURKB; aurora kinase B ; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; STK12; aurkb-sv1; aurkb-sv2; aurora-1; aurora-B; aurora kinase B-Sv1; aurora kinase B-Sv2; serine/threonine kinase 12; EC 2.7.11.1; Aurora- and Ipl1-like midbody-associated protein 1; Aurora-related kinase 2; Aurora/IPL1-related kinase 2
Gene ID : 9212
mRNA Refseq : NM_004217
Protein Refseq : NP_004208
MIM : 604970
UniProt ID : Q96GD4
Chromosome Location : 17p13.1
Function : ATP binding; magnesium ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity

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