||Recombinant human Flt-3 fused at the N-terminus to a GST-His6 thrombin cleavage site sequence was expressed inS. frugiperdainsect cells using a baculovirus expression system. MW = 82076 Da.
||Flt-3 belongs to the class III receptor tyrosine kinase family that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in vitro in cooperation with other cytokines. Flt-3 is also expressed in the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells.
||Liquid. In 100 mM NaCl, 50 mM Tris-HCl, 15 mM reduced glutathione, 5 mM DTT, 20% glycerol, pH 8.0. Please refer to vial label for lot-specific concentration.
||One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Glu,Tyr)4:1 per min at 30°C, using variable concentrations of ATP (0.1-12.6 µM).
||≥28 U/mg protein.
||Standard Handling (MSDS available upon request).
||≤ -70°C. Avoid freeze/thaw Following initial thaw, aliquot and freeze (-70°C).