Active Recombinant Human LCK, His-tagged

• Cat.No.: LCK-692H
• Product Overview: Recombinant, human Lck fused to a His Tag sequence and expressed inS. frugiperda insect cells.Lck is a member of the Src tyrosine kinases that plays a role in T cell activation and in the regulation of mitochondrial apoptosis pathways.

Specification

• Species: Human
• Source: S.frugiperda
• Tag: His
• Description: LCK (p56lck) is a member of the src family of non-receptor tyrosine kinases. It was identified as a gene rearranged and overexpressed in the murine lymphoma LSTRA, most likely as a result of the insertion of Moloney murine leukemia virus DNA immediately adjacent to the gene. Lck behaves as a proto-oncogene and can lead to cell transformation upon activation. A number of human cancer cell lines show overexpression of LCK, pointing to a possible role for this kinase in the initiation and maintenance of the transformed state in human cancers.
• Form: Liquid.
• Formulation: In 100 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 50 μM EDTA, 50% glycerol, 0.05% NP-40, pH 7.5.
• Molar Mass: 60500 Da.
• Specific Activity: ≥100 U/mg protein.
• Unit Definition: One unit is defined as the amount of enzyme that will transfer 1.0 nmol phosphate to poly (Glu,Tyr) 4:1 substrate per min at 30°C, pH 7.5.
• Purity: ≥90% by SDS-PAGE.
• Storage: ≤ -70oC. Ship: Dry Ice Only.

Gene Information

• Gene Name: LCK lymphocyte-specific protein tyrosine kinase [ Homo sapiens ]
• Synonyms:
  LCK; lymphocyte-specific protein tyrosine kinase; YT16; p56lck; pp58lck; protein tyrosine kinase; protein tyrosine kinase; protein tyrosine kinase; protein tyrosine kinase; EC 2.7.10.2; LSK; p56-LCK; Lymphocyte cell-specific protein-tyrosine kinase; T cell-specific protein-tyrosine kinase
• Gene ID: 3932
• mRNA Refseq: NM_001042771
• Protein Refseq: NP_001036236
• MIM: 153390
• UniProt ID: P06239
• Chromosome Location: 1p34.3
• Pathway: Natural killer cell mediated cytotoxicity; Primary immunodeficiency; T cell receptor signaling pathway; HIV Infection; HIV Infection; Signaling in Immune system
• Function: non-membrane spanning protein tyrosine kinase activity; ATP binding; ATPase binding; CD4 receptor binding; CD8 receptor binding; SH2 domain binding; glycoprotein binding; nucleotide binding; phosphoinositide 3-kinase binding; protein C-terminus binding; protein kinase binding; protein serine/threonine phosphatase activity; transferase activity