||Recombinant Human Cyclin-Dependent Kinase Inhibitor 3 with a GST tag is expressed inE. coli.
||CDKN3 is a member of the dual specificity protein phosphatase family that acts as a cyclin-dependent kinase inhibitor. CDKN3 has been shown to interact with and dephosphorylate specifically the CDK2 kinase thereby preventing it's activation. CDKN3 has been reported to be deleted, mutated, or overexpressed in several kinds of cancers. Increased expression of CDKN3 leads to increased levels of kinase-associated phosphatase activity that inhibits the G(1)/S transition of the cell cycle by dephosphorylating the cyclin-dependent kinases.
||Greater than 90 % pure as determined by SDS-PAGE analysis.
||50 kDa bandby SDS-PAGE analysis.
||The recombinant Cdc25C (catalytic domain) is supplied frozen in a buffer containing 20mM Tris-HCl (pH 8.2), 1mM EDTA, 2 mM DTT and 50% glycerol.
||Stable for 12 months at -70°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot enzyme toavoid repeated freezing and thawing.