||Recombinant human Androgen Receptor with a His tag is expressed inE. coli.
||Signaling by the androgen receptor plays a key role in proper development and function of male reproductive organs. The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. The androgen receptor (AR) has been shown to play a critical role in the development and progression of the prostate cancer.
||Sterile filtered liquid containing 6M Urea + 20 mM Sodium Phosphate + 0.5 M NaCl +0.5 M Imidazole, pH 7.4.
||>95.0% as determined by reducing and non-reducing SDS-PAGE Silver Stained gel.
||Stable for 6 months in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.