Active Recombinant Mouse Hmgb1 protein, His-tagged

• Cat.No.: Hmgb1-2628M
• Product Overview: Recombinant Mouse Hmgb1(Met1-Glu215) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Sterile liquid; 20 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 1 mM DTT. 0.22 um filtered.
• Bio-activity: The ED50 of this protein, as measured by mouse TNF alpha induction in RAW264.7 cells, is 3-25 ug/mL in the presence of 10 ug/mL Polymyxin B. This corresponds to a specific activity of 3,300-40 Units/mg.
• Molecular Mass: Has a predicted molecular mass of 27.6 kDa.
• Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
• Purity: > 98%, as determined by SDS-PAGE
• Storage: Store at less than or equal to -70 centigrade.
• Concentration: 0.5 mg/mL
• Publication:
  Functional Role of Soluble Receptor for Advanced Glycation End Products in Stroke (2013)

Gene Information

• Gene Name: Hmgb1 high mobility group box 1 [ Mus musculus ]
• Official Symbol: Hmgb1
• Synonyms: HMGB1; high mobility group box 1; high mobility group protein B1; high mobility group protein 1; DEF; p30; Hmg1; HMG-1; SBP-1; amphoterin; MGC103168; MGC103169; MGC117896; MGC117897
• Gene ID: 15289
• mRNA Refseq: NM_010439
• Protein Refseq: NP_034569
• UniProt ID: P63158
• Chromosome Location: 5 G3; 5 89.18 Cm
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Activation of DNA fragmentation factor, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis induced DNA fragmentation, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; Base excision repair, organism-specific biosystem
• Function: 5S rRNA binding; DNA binding; DNA binding, bending; RAGE receptor binding; bent DNA binding; binding; calcium-dependent protein kinase regulator activity; crossed form four-way junction DNA binding; cytokine activity; cytokine activity; double-stranded DNA binding; four-way junction DNA binding; glycolipid binding; heparin binding; open form four-way junction DNA binding; peptide binding

Reviews

09/22/2021

Broad storage conditions of HMGB1ins make the experimental process more stable and reliable, which provides a strong guarantee for scientific research.

05/02/2020

I found that tHMGB1 exhibited very good stability during storage, which allowed us to perform experiments easily and obtain reliable results.

01/11/2019

The anti-contamination ability of HMGB1 was excellent in Western blot, and the contamination problem in the experiment was avoided.

Q&As

Why is HMGB1 protein associated with tumors?

This protein can promote tumor growth, invasion and angiogenesis, and plays an important role in tumor immune evasion.

Is HMGB1 protein involved in tumor metastasis?

Yes, HMGB1 protein plays an important role in tumor metastasis, particularly by promoting invasion of tumor cells and migration of the hematopoietic system.

What is the association between HMGB1 protein and cardiovascular disease?

Is involved in inflammatory response, vascular damage, and thrombosis in cardiovascular disease and may be associated with myocardial ischemia-reperfusion injury.

What is the effect of HMGB1 protein on the immune system?

HMGB1 protein can promote the activation, chemotaxis and inflammatory response of immune cells, and is also involved in regulating the balance of autoimmune response.

What role does HMGB1 play in diabetes?

HMGB1 protein is associated with processes such as chronic inflammation and insulin resistance in diabetes and may be a potential target for treatment and prevention.

Does HMGB1 play a role in liver disease?

The protein is involved in pathological processes such as liver fibrosis, hepatitis, and liver cancer in liver disease and may be a target for therapeutic strategies.