Recombinant Human GSK3A, GST-tagged
Cat.No. : | GSK3A-2648H |
Product Overview : | Recombinant Human GSK3A wasfused at the N-terninus to GST and expressed inS. frugiperda insect cellsusing a baculovirus expression system. MW = 81500 Da. |
- Specification
- Gene Information
- Related Products
Cat. No. : | GSK3A-2648H |
Description : | GSK3α is unique in its preference for a priming phosphate at n+4 (where n is the site of phosphorylation by GSK3α) in order to phosphorylate many of its substrates. This motif is found in several well-established substrates of GSK3α, such as glycogen synthase, the epsilon subunit of eIF2B, and ATP citrate-lyase. The active kinase is useful in the study of GSK3α regulation and for inhibitor screening. |
Source : | S. frugiperda. |
Purity : | ≥80% by SDS-PAGE. |
Form : | Liquid. In 100 mM NaCl, 25 mM Tris-HCl, 10 mM reduced glutathione, 3 mM DTT, 50% glycerol, 0.05% Tween-20 detergent, pH 8.0. |
Specific Activity : | ≥700 units/mg protein. |
Unit Definition : | One unit is defined as the amount of enzyme that will phosphorylate 1 nmol glycogen synthase peptide substrate (YRRAAVPPSPSLSRHSSPHQSEDEEE) per min at 30°C, pH 7.4. |
Toxicity : | Standard Handling (MSDS available upon request). |
Recommended Reaction Conditions : | 50 mM HEPES, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 100 µM ATP, 20 µM peptide substrate (YRRAAVPPSPSLSRHSSPHQSEDEEE), 3 µM sodium orthovanadate, and 100 µg enzyme. |
Storage : | ≤ -70℃. Avoid freeze/thaw. Following reconstitution, aliquot and freeze (-70°C). |
Gene Name : | GSK3A glycogen synthase kinase 3 alpha [ Homo sapiens ] |
Synonyms : | GSK3A; glycogen synthase kinase 3 alpha; DKFZp686D0638; Glycogen synthase kinase-3 alpha; GSK-3 alpha; EC 2.7.11.26 |
Gene ID : | 2931 |
mRNA Refseq : | NM_019884 |
Protein Refseq : | NP_063937 |
MIM : | 606784 |
UniProt ID : | P49840 |
Chromosome Location : | 19q13 |
Pathway : | Chemokine signaling pathway; Signalling by NGF |
Function : | ATP binding; protein binding; nucleotide binding; transferase activity; glycogen synthase kinase 3 activity; protein kinase A catalytic subunit binding; tau-protein kinase activity |
Products Types
◆ Recombinant Protein | ||
GSK3A-4397H | Recombinant Human GSK3A Protein, GST-tagged | +Inquiry |
GSK3A-0315H | Recombinant Human GSK3A Protein (S2-S483), Tag Free | +Inquiry |
Gsk3a-3317M | Recombinant Mouse Gsk3a Protein, Myc/DDK-tagged | +Inquiry |
GSK3A-1805R | Recombinant Rhesus Macaque GSK3A Protein, His (Fc)-Avi-tagged | +Inquiry |
GSK3A-055H | Recombinant Human GSK3A Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
◆ Lysates | ||
GSK3A-5723HCL | Recombinant Human GSK3A 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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