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Recombinant Human Pyrophosphatase (Inorganic) 1, T7-tagged

Cat. No.: ARF6-3369H
Product Overview: Recombinant human ARF6 protein , fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 22.2 kDa (195aa).
Description: ADP-ribosylation factor 6, also known as ARF6, is a member of the ADP ribosylation factor family of GTP-binding proteins. It is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. Additionally, ARF6 play a central role in maintenance of organelle integrity, assembly of coat proteins and activation of phospholipase D.
Source: E.coli.
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 0.2mM PMSF.
Purity: > 95% by SDS - PAGE.
Concentration: 1.0 mg/ml (determined by Bradford assay).
Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MGKVLSKIFG NKEMRILMLG LDAAGKTTIL YKL KLGQSVT TIPTVGFNVE TVTYKNVKFN VWDVGGQDKIRPLWRHYYTG TQGLIF VV DC ADRDRIDEAR QELHRIINDR EMRDAIILIF ANKQDLPDAM KPHEIQEKLG LTRIRDRNWY VQPSCATSGDGLYEGLTWLT SNYKS.
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Fuction: GTP binding; GTPase activity; nucleotide binding; protein binding; thioesterase binding
Pathways: Endocytosis; Fc gamma R-mediated phagocytosis
Gene Name: ARF6 ADP-ribosylation factor 6 [ Homo sapiens ]
Synonyms: ADP-ribosylation factor 6; DKFZp564M0264; ARF6
Gene ID: 382
mRNA Refseq: NM_001663
Protein Refseq: NP_001654
MIM: 600464
UniProt ID: P62330
Chromosome Location: 14q21.3

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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