Recombinant Equine IL4 IL4-256E

Recombinant Equine IL4

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Recombinant Equine IL4

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Cat.No. : IL4-256E
Product Overview : Equine IL-4 was produced in yeast and therefore does not have endotoxin, is naturally folded, and post-translationally modified.
Description : Interleukin-4 (IL-4) induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4. IL-4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is a key regulator in humoral and adaptive immunity. IL-4 induces B-cell class switching to IgE, and up-regulates MHC class II production. Overproduction of IL-4 is associated with allergies.
Source : Yeast
Species : Equine
Form : Lyophilized without carrier protein.
Bio-activity : The biological activity of recombinant equine IL-4 was measured in a cell proliferation assay using the human TF-1 cell line. The ED50 for this effect is typically 40-60 ng/mL.
Molecular Mass : Equine IL-4 has a predicted molecular weight of 12.8 kDa.
AA Sequence : GCKYDITLQE IIKTLNNLTD GKGKNSCMEL TVADAFAGPK NTDGKEICRA AKVLQQLYKR HDRSLIKECL SGLDRNLKGM ANGTCCTVNE AKKSTLKDFL ERLKTIMKEK YSKC (114)
Purity : >95% as visualized by SDS-PAGE analysis.
Applications : The Equine IL-4 protein can be used in cell culture, as an IL-4 ELISA Standard, and as a Western Blot Control.
Storage : Stable for up to twelve months from date of receipt at -20oC. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20oC. Avoid repeated freeze/thaw cycles.
Reconstitution : Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.
Gene Name : IL4 interleukin 4 [ Equus caballus (horse) ]
Official Symbol : IL4
Synonyms : IL4; interleukin 4; IL-4; BSF-1; interleukin-4; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1
Gene ID : 100034225
mRNA Refseq : NM_001082519
Protein Refseq : NP_001075988
UniProt ID : P42202
Chromosome Location : 14
Function : Allograft rejection; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction

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