||Recombinant human NHP2L1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography.MW = 16.7 kDa(152aa).
||NHP2L1 belongings to the ribosomal protein L7Ae family. This protein localizes to the nucleus, mainly concentrated in the dense fibrillar component of the nucleolus. It directly binds to the 5" stem-loop of U4 snRNA and plays an important role in the late stage of spliceosome assembly, prior to step I of splicing catalysis.
||Liquid. In 20 mM Tris-HCl Buffer (pH 7.5) containing 100 mM NaCl, 10% Glycerol.
||> 95% by SDS - PAGE.
||0.25 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MGSHMTEADV NPKAYPLADA HLTKKLLDLV QQS CNYKQLR KGANEATKTL NRGISEFIVM AADAEPLEIILHLPLLCEDK NVPYVFVRSK QALGRACGVS RPVIACSVTI KEGSQLKQQI QSIQQSIERL LV.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Spliceosome; Gene Expression; mRNA Processing