||Recombinant human NIT2 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 33 kDa (299aa).
||Omega-amidase NIT2, also known NIT2, belongs to the nitrilase superfamily. This protein has an omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Also Nit2, which is widely distributed in nature, has been suggested to be a tumor suppressor protein.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl.
||> 95% by SDS - PAGE.
||1 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MGSMTSFRLA LIQLQISSIK SDNVTRACSF IREA ATQGAK IVSLPECFNS PYGAKYFPEY AEKIPGESTQKLSEVAKECS IYLIGGSIPE EDAGKLYNTC AVFGPDGTLL AKYRKIHLFD IDVPGKITFQ ESKTLSPGDS FSTF DTPYCR VGLGICYDMR FAELAQIYAQ RGCQLLVYPG AFNLTTGPAH WELL QRS RAV DNQVYVATAS PARDDKASYV AWGHSTVVNP WGEVLAKAGT EEAI VYSDI DLKKLAEIRQQ IPVFRQKRSD LYAVEMKKP.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Alanine, aspartate and glutamate metabolism