||Recombinant human 5-Lipoxygenase expressed inS. fruigiperdainsect cells using a baculovirus overexpression system. It is localized in the cytosol of resting neutrophils, and is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA2.
||One unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25°C in 50 mM Tris-HCl, 100 µM arachidonate, 2 mM CaCl2, 1 mM ATP, 250 µM oxygen. Oxygen consumption can be measured using a oxygraph equipped with a Clark oxygen electrode.
||Liquid In 100 mM Tris containing 5 mM EGTA, pH 8.0.
||Standard Handling (MSDS available upon request).
||≤-70°C. Avoid freeze/thaw Following initial thaw, aliquot and freeze (-70°C).