Recombinant Human Arachidonate 5-lipoxygenase 1
Cat.No. : | ALOX5-723H |
- Specification
- Gene Information
- Related Products
Cat. No. : | ALOX5-723H |
Description : | Recombinant human 5-Lipoxygenase expressed inS. fruigiperdainsect cells using a baculovirus overexpression system. It is localized in the cytosol of resting neutrophils, and is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA2. |
Source : | S. fruigiperda. |
Molar Mass : | 78000. |
Specific Activity : | ~20 units/mg. |
Unit Definition : | One unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25°C in 50 mM Tris-HCl, 100 µM arachidonate, 2 mM CaCl2, 1 mM ATP, 250 µM oxygen. Oxygen consumption can be measured using a oxygraph equipped with a Clark oxygen electrode. |
Form : | Liquid In 100 mM Tris containing 5 mM EGTA, pH 8.0. |
Toxicity : | Standard Handling (MSDS available upon request). |
Storage : | ≤-70°C. Avoid freeze/thaw Following initial thaw, aliquot and freeze (-70°C). |
Gene Name : | ALOX5 arachidonate 5-lipoxygenase [ Homo sapiens ] |
Synonyms : | arachidonate 5-lipoxygenase; ALOX5; 5-LO; 5LPG; LOG5; 5-LOX; MGC163204; leukotriene A4 synthase; arachidonic acid 5-lipoxygenase |
Gene ID : | 240 |
mRNA Refseq : | NM_000698 |
Protein Refseq : | NP_000689 |
MIM : | 152390 |
UniProt ID : | P09917 |
Chromosome Location : | 8p12 |
Pathway : | Arachidonic acid metabolism; Metabolic pathwaysy |
Function : | arachidonate 5-lipoxygenase activity; iron ion binding; lipoxygenase activity; metal ion binding; oxidoreductase activity; protein binding |
Products Types
◆ Recombinant Protein | ||
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◆ Lysates | ||
ALOX5-8896HCL | Recombinant Human ALOX5 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (13)
Ask a questionThe ALOX5 coding gene is located on human chromosome 10.
Currently known ALOX5 inhibitors include trypanosomamine, Ritopane, MK-886, etc., whose mechanism of action is to interfere with the expression or function of ALOX5 in different ways, thereby playing an anti-inflammatory or anti-tumor role.
ALOX5 is involved in a variety of biological processes, including apoptosis, cell proliferation, inflammatory response, and cancer metastasis.
The gene expression can be regulated by a variety of factors such as prostaglandins, cytokines and interferons.
Some foods rich in polyphenols, such as cucumbers, tomatoes, grapes, green tea, etc., are thought to inhibit ALOX5 activity.
In general, the ALOX5 gene is highly expressed in eosinophils and monocytes.
The ALOX5 promoter region is the starting point of gene transcription and is responsible for mediating the transcription regulation of the ALOX5 gene.
Drugs that block the ALOX5 signaling pathway, such as Zileuton, MK886, and A861, have been shown to inhibit tumor cell proliferation and invasion. Currently, there are also many researchers who are exploring ALOX5 in tumors.
ALOX5 gene mutation may cause asthma, allergic rhinitis, eczema and other allergic diseases.
5-HETE is one of the representative products of ALOX5 catalyzed synthesis, and is an activator of platelets and neutrophils.
There is no definite answer to this question. Current studies believe that ALOX5 can affect the occurrence, development and metastasis of tumors through multiple ways, including regulating the proliferation, apoptosis, invasion and metastasis of tumor cells.
ALOX5 knockout mouse models can be used to study a variety of biological processes and diseases, such as immune regulation, inflammation, asthma, eczema, autoimmunity, etc.
At present, the commonly used methods include real-time fluorescent quantitative PCR, Northern blot, Western blot, etc.
Customer Reviews (3)
Write a reviewAfter ALOX5 application, the background of the Western blot was very clean with no nonspecific signal.
ALOX5 significantly improved the experimental accuracy and made the experimental results more reliable.
I appreciate the stability of ALOX5, which can be kept at room temperature for several days without spoilage.
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