||Recombinant rat adiponectin encoding the mature peptide of rat adiponectin (amino acid His16~Asn244) was tagged with FLAG at the N-terminus. The tagged protein was expressed in a human embryonic kidney cell line,HEK293,and purified from conditioned media.
||Adiponectin, also known as apM1, Acrp30, GBP28 and adipoQ, is a circulating hormone predominantly produced from adipose tissue. Many pharmacological studies demonstrated that this protein possesses potent anti-diabetic, anti-atherogenic and anti-inflammatory functions. Supplement of adiponectin protein can decrease blood glucose, improve insulin sensitivity, alleviate fatty liver and prevent atherosclerosis. The protein is post-translationally modified by hydroxylation and glycosylation, and forms three different oligomeric complexes in the circulation.
||0.2 m filtered solution in phosphate-buffered saline.
||effect on the phosphorylation of acetyl-CoA carboxylase in C2C12 cells.
||>95%, as determined by SDS-PAGE.
||<0.1EU/g, as determined by the LAL method.
|Stability Storage And Handling:
||The protein solution should be stored in working aliquots at -20°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.