Recombinant Mouse Map2k1, GST-tagged


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Recombinant Mouse Map2k1, GST-tagged

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Cat.No. : Map2k1-666M
Product Overview : Recombinant full-length mouse MEK1 (S218E, S222E) was expressed in E. coli cells using a N-terminal GST tag.
Description : MEK1 (EE) is a member of the dual specificity protein kinase family that acts as a mitogen-activated protein kinase (MAPK) kinase. MEK1 lies upstream of MAPK/ERK and stimulates the enzymatic activity of MAPK/ERK upon a wide variety of extra- and intracellular signals. As an essential component of MAPK/ERK signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Constitutive activation of MEK1 results in cellular transformation. Thus, MEK1 represents a likely target for pharmacologic intervention in proliferative disease such as cancer.
Source : E.coli
Species : Mouse
Tag : GST
Form : Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Bio-activity : 326 nmol/min/mg
Molecular Mass : ~69 kDa
Protein length : Full Length
Purity : >70%
Applications : Kinase Assay, Western Blot
Stability : 1 yr at -70°C from date of shipment.
Storage : Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration : 0.1 ug/ul
Gene Name : Map2k1 mitogen-activated protein kinase kinase 1 [ Mus musculus (house mouse) ]
Official Symbol : Map2k1
Synonyms : Map2k1; mitogen-activated protein kinase kinase 1; Mek1; MEKK1; MAPKK1; Prkmk1; dual specificity mitogen-activated protein kinase kinase 1; MEK 1; MAPKK 1; MAPK/ERK kinase 1; MAP kinase kinase 1; ERK activator kinase 1; mitogen activated protein kinase kinase 1; protein kinase, mitogen activated, kinase 1, p45; NP_032953.1; EC
Gene ID : 26395
mRNA Refseq : NM_008927
Protein Refseq : NP_032953
UniProt ID : P31938
Chromosome Location : 9 C; 9 34.55 cM
Pathway : ARMS-mediated activation; Activated TLR4 signalling; Acute myeloid leukemia; Androgen Receptor Signaling Pathway
Function : ATP binding; MAP kinase kinase activity; mitogen-activated protein kinase kinase kinase binding

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