||Recombinant human DDT, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW = 14.8 kDa(138aa).
||DDT is an enzyme that catayzes the tautomerization of D-dopachrome to give 5,6-dihydroxyindole (DHI). This protein belongs to the family oflyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33% identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity. In addition, it may functions a proinflammatory cytokine.
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MPFLELDTNL PANRVPAGLE KRLCAAAASI LGKPADRVNV TVRPGLAMAL SGSTEPCAQL SISSIGVVGT AEDNRSHSAH FFEFLTKELA LGQDRILIRF FPLESWQIGK IGTVMTFL
||> 95% by SDS – PAGE.
||Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol.
||1 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.