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Active Recombinant Mouse Eng protein, His-tagged

Cat.No. : Eng-3289M
Product Overview : Recombinant Mouse Eng protein(NP_031958.2)(Met1-Gly580), fused with His tag, was expressed in HEK293.
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Source : HEK293
Species : Mouse
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity : 1. Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. The ED50 for this effect is typically 25-100 ng/mL in the presence of 2 ng/mL of recombinant human BMP9.
Molecular Mass : The secreted recombinant mouse CD105 consists of 565 amino acids and has a calculated molecular mass of 61.2 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 65-70 kDa in SDS-PAGE under reducing conditions.
Proteinlength : Met1-Gly580
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 97 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Eng endoglin [ Mus musculus ]
Official Symbol : Eng
Synonyms : ENG; endoglin; transmembrane glycoprotein; cell surface MJ7/18 antigen; CD105; AI528660; AI662476; S-endoglin;
Gene ID : 13805
mRNA Refseq : NM_001146348
Protein Refseq : NP_001139820

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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