Active Recombinant Human NOG protein(Met1-Cys232), His-tagged
Cat.No. : | NOG-3156H |
Product Overview : | Recombinant Human Noggin precursor (NP_005441.1) (Met 1-Cys 232) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus. |
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Source : | HEK293 |
Species : | Human |
Tag : | C-His |
Protein length : | Met1-Cys232 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured by its ability to inhibit recombinant human BMP4-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 0.05-0.3 μg/mL in the presence of 25 ng/mL of recombinant human BMP4. |
Molecular Mass : | The recombinant human Noggin comprises 216 amino acids after removal of the signal peptide and has a predicted molecular mass of 24.6 kDa. As a result of glycosylation, rhNoggin migrates as an approximately 30 kDa bnd in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | NOG noggin [ Homo sapiens ] |
Official Symbol : | NOG |
Synonyms : | NOG; noggin; SYM1, symphalangism 1 (proximal) , synostoses (multiple) syndrome 1 , SYNS1; symphalangism 1 (proximal); SYM1; SYNS1; |
Gene ID : | 9241 |
mRNA Refseq : | NM_005450 |
Protein Refseq : | NP_005441 |
MIM : | 602991 |
UniProt ID : | Q13253 |
Products Types
◆ Recombinant Protein | ||
NOG323P | Recombinant Pig NOG Protein, His-tagged | +Inquiry |
Nog-322M | Recombinant Mouse Nog Protein, His/GST-tagged | +Inquiry |
Nog-325R | Recombinant Rat Nog Protein, His/MBP-tagged | +Inquiry |
NOG-319D | Recombinant Dog NOG Protein, His-tagged | +Inquiry |
NOG-315H | Active Recombinant Human NOG Protein (Gln28-Cys232), C-Fc tagged, Animal-free, Carrier-free | +Inquiry |
◆ Lysates | ||
NOG-2414HCL | Recombinant Human NOG cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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