||Recombinant human RhoA protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 25.7 kDa(227aa).
||RhoA belongs to the small GTPase superfamily, Rho family, proteins involved in the regulation and timing of cell division. This protein is a small GTPase protein to regulate the actin cytoskeleton in the formation of stress fibers and acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila).
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl,1mM DTT.
||> 90% by SDS - PAGE.
||1.0 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAA IRKKLVIVGD GACGKTCLLI VFSKDQFPEV YVPTVFENYV ADIEVDGKQV ELALWDTAGQ EDYDRLRPLS YPDTDVILMC FSIDSPDSLE NIPEKWTPEV KHFCPNVPII LVGNKKDLRN DEHTRRELAK MKQEPVKPEE GRDMANRIGA FGYMECSAKT KDGVREVFEM ATRAALQARR GKKKSGC.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Adherens junction; Axon guidance; Chemokine signaling pathway; Colorectal cancer; Leukocyte transendothelial migration; Neurotrophin signaling pathway; Pathogenic Escherichia coli infection; Regulation of actin cytoskeleton; T cell receptor signaling pathway; TGF-beta signaling pathway; Tight junction; Vascular smooth muscle contraction; Wnt signaling pathway; Signaling by Rho GTPases; Signaling by GPCR; Signalling by NGF