Recombinant Human Ras Homolog Gene Family, His-tagged

• Cat.No.: RHOA-3372H
• Product Overview: Recombinant human RhoA protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 25.7 kDa(227aa).

Specification

• Cat. No.: RHOA-3372H
• Description: RhoA belongs to the small GTPase superfamily, Rho family, proteins involved in the regulation and timing of cell division. This protein is a small GTPase protein to regulate the actin cytoskeleton in the formation of stress fibers and acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila).
• Source: E.coli.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl,1mM DTT.
• Purity: > 90% by SDS - PAGE.
• Concentration: 1.0 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAA IRKKLVIVGD GACGKTCLLI VFSKDQFPEV YVPTVFENYV ADIEVDGKQV ELALWDTAGQ EDYDRLRPLS YPDTDVILMC FSIDSPDSLE NIPEKWTPEV KHFCPNVPII LVGNKKDLRN DEHTRRELAK MKQEPVKPEE GRDMANRIGA FGYMECSAKT KDGVREVFEM ATRAALQARR GKKKSGC.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Adherens junction; Axon guidance; Chemokine signaling pathway; Colorectal cancer; Leukocyte transendothelial migration; Neurotrophin signaling pathway; Pathogenic Escherichia coli infection; Regulation of actin cytoskeleton; T cell receptor signaling pathway; TGF-beta signaling pathway; Tight junction; Vascular smooth muscle contraction; Wnt signaling pathway; Signaling by Rho GTPases; Signaling by GPCR; Signalling by NGF
• Publication:
  Utility of Bioluminescent Homogeneous Nucleotide Detection Assays in Measuring Activities of Nucleotide-Sugar Dependent Glycosyltransferases and Studying Their Inhibitors (2021)

Gene Information

• Gene Name: RHOA ras homolog gene family, member A [ Homo sapiens ]
• Synonyms: RHOA; ras homolog gene family, member A; ARHA; ARH12; RHO12; RHOH12; oncogene RHO H12; Aplysia ras-related homolog 12; small GTP binding protein RhoA
• Gene ID: 387
• mRNA Refseq: NM_001664
• Protein Refseq: NP_001655
• MIM: 165390
• UniProt ID: P61586
• Chromosome Location: 3p21.3
• Function: GTP binding; GTPase activity; magnesium ion binding; myosin binding; nucleotide binding; protein binding