||Recombinant HumanKAT2B is expressed inbaculovirussystem and purified by an affinity column in combination with FPLC chromatography. Molecular Weight: 91 kDa.
||P300/CBP-associated factor (PCAF), also known as K(lysine) acetyltransferase 2B (KAT2B), is a human gene and trancriptional coactivator associated with p53.
||1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 0.2 mM EDTA and 1mM DTT.
||The purified protein is greater than 95% homogeneous and contains no detectable proteases, DNase and RNase activity.
||1 unit equals 1 nanogram of purified protein. 1-5 units are sufficient for a gel mobility shift assay in a 20µl reaction; 50-100 units are sufficient for reconstituted transcription assay and 100-200 units are sufficient for a protein-protein interaction assay or an acetylation assay.
||Recombinant PCAF can be used 1) for protein-protein interaction assay; 2) for in vitro transcription assay; 3) for in vitro acetylation assay; and 4) for cell growth assay.
||Store at -80°C.