Recombinant Human CST4 protein, His-tagged CST4-3212H

Recombinant Human CST4 protein, His-tagged

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Recombinant Human CST4 protein, His-tagged

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Cat.No. : CST4-3212H
Product Overview : Recombinant Human CST4 (Ser21-Ala141) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Cystatin-4 (CST4) is also known as Cystatin-S, Salivary acidic protein 1, which is a secreted protein, which belongs to the cystatin family. Cystatin-S is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level) and is also expressed in saliva, tears, urine and seminal fluid. Cystatin-4/Cystatin S strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Ser21
Form : Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH6.0, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 15 kDa. The protein migrates as 15 kDa under reducing (R) condition (SDS-PAGE).
Protein length : Ser21-Ala141
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CST4-3212H :

Human Cystatin S, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : CST4
Official Symbol : CST4
Synonyms : CST4; cystatin S; cystatin-S; cystatin 4; cystatin-4; cystatin-SA-III; salivary acidic protein 1; MGC71923
Gene ID : 1472
mRNA Refseq : NM_001899
Protein Refseq : NP_001890
MIM : 123857
UniProt ID : P01036

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