Active Recombinant Mouse Pilra protein(Met1-Val197), His-tagged

• Cat.No.: Pilra-3358M
• Product Overview: Recombinant Mouse PILRA (NP_705730.1) extracellular domain (Met 1-Val 197) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Val197
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PILRA at 20 μg/ml (100 μl/well) can bind mouse CD99. The EC50 of mouse CD99 is 1.55 μg/ml.
• Molecular Mass: The recombinant mouse PILRA consists of 177 amino acids and has a predicted molecular mass of 20.2 kDa. As a result of glycosylation, the apparent molecular mass of rm PILRA is approximately 35-45 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Pilra paired immunoglobin-like type 2 receptor alpha [ Mus musculus ]
• Official Symbol: Pilra
• Synonyms: PILRA; paired immunoglobin-like type 2 receptor alpha; paired immunoglobulin-like type 2 receptor alpha; cell surface receptor FDF03; inhibitory receptor PILR-alpha; FDF03; AV021745
• Gene ID: 231805
• mRNA Refseq: NM_153510
• Protein Refseq: NP_705730